Nevertheless, we found no significant difference in the percentage of OLIG2+/NKX2.2+ tNPCs between euploid and trisomic lines in either isogenic pair in the Wnt-C59 condition (p = 0.30 WC-24-02-DS and p = 0.57 ILD; Figure 3D; Supplementary Table 4). Here, NKX2-2 is linked to interstitial lung disease.