At young age (2-mo) Pcyt2+/− have no clinical symptoms of NAFLD, However, 2-mo Pcyt2+/− exhibit early defects in fatty acid metabolism that favour FA synthesis and persist into adulthood, Adult (6-8mo) Pcyt2+/− exhibit a fasting-specific deficit in Pi3k/Akt signalling with a shift to increased glucose production by gluconeogenesis, and reduced lipolysis and FA oxidation 20. Here, PCYT2 is linked to metabolic dysfunction-associated steatotic liver disease.