In contrast, the ZIKV infection at MOI of 1 significantly increased the extracellular HMGB1 levels at 48 h.p.i (1074.75 ± 91.25 pg/mL, P < 0.001) and 72 h.p.i (1397.25 ± 13.75 pg/mL, P < 0.001), in comparison to those of the mock-infected cells (Fig. 3a). The gene discussed is HMGB1; the disease is Zika virus infectious disease.