We observed increased insulin sensitivity (increase in AOC for the IPITT) in 100% (7 out of 7) of the HFD-fed skmSTXtg mice after 4 weeks of Dox induction (Fig. 2b, p = 0.02), demonstrating that skeletal muscle STX4 can remediate insulin resistance in HFD-fed mice, despite the continued intake of a diabetogenic diet. This evidence concerns the gene STX4 and Insulin resistance.