Regardless of the repression of AD-associated genes, mhCOs showed a similar expression of the microglia markers Tmem119 and Iba1 (Supplementary Fig. 8e) and presented with a similar number CD11b+ microglia-like cells (Fig. 5c), suggesting a successful formation of cortical organoids with microglia. This evidence concerns the gene ITGAM and Alzheimer disease.