Direct MFV analysis of circulating EVs in human plasma samples (Table 1, N = 89) revealed elevated ACE2+ EVs in the plasma of COVID-19 patients in comparison to seronegative controls, with a more dramatic increase in the acute phase and a modest elevation in the convalescent-phase (Fig. 1a, b, Supplementary Fig. 1a–d), with the latter in association with COVID-19 severe disease showing relatively higher levels in inpatient samples (Supplementary Fig. 1b). The gene discussed is ACE2; the disease is COVID-19.