Moreover, BAPTA-AM, instead of EGTA, inhibited the elevation of the NADP+/NADPH ratio in AML-12 cells after treatment with AuNPs and LPS-conditioned medium, further suggesting that extracellular ROS increases intracellular calcium concentrations to activate NOX2 in AML-12 cells (Fig. 8F). The gene discussed is CYBB; the disease is acute myeloid leukemia.