In order to evaluate the influence of PFKFB3 on RCC progression, this study initially employed RT-qPCR and Western blot to detect transcriptomic and proteomic PFKFB3 levels in an immortalized, normal human-proximal-tubule-epithelial cell line (HK-2), together with a panel of RCC cell lines (ACHN, Caki-2, A498, 786–0, OS-RC-1). Here, PFKFB3 is linked to renal cell carcinoma.