As cleavage of GSDMD is induced by cleaved caspase-1, leading to pyroptosis in DCM [16], we further determined the protein levels of cleaved GSDMD (Figure 5E) and mRNA levels of Gsdmd (Figure 5F), both of which were increased in the diabetic hearts and were decreased by KO (Figure 5E,F). Here, CASP1 is linked to familial dilated cardiomyopathy.