By using high-throughput NGS and bioinformatics, we identified a panel of six 2′-F-Py RNA aptamers able to bind to human TNBC cell lines (MDA-MB-231, BT-549, MDA-MB-436, DU4475, MDA-MB-468) covering different TNBC subtypes, and to distinguish them from both normal cells and non-TNBC breast cancer cell lines representative of luminal A (MCF-7 and T47D) and HER2-positive (SK-BR-3) molecular categories. Here, ERBB2 is linked to breast cancer.