The cytotoxic antitumor effect of the live streptococci S. pyogenes 7, S. pyogenes 21, S. pyogenes GUR and S. pyogenes GURSA1 as well as the cytotoxic effect of NGF, LL-37, and PG-1 on the rat glioma C6 cell culture was demonstrated using the MTT assay, the trypan blue exclusion test of cell viability and the xCELLigence system for real-time cytotoxicity analysis. Here, NGF is linked to central nervous system cancer.