All these changes suggest a complex multi-organ function compromise in animals fed a FRD: adipose tissue (evidenced by increased free fatty acids levels), liver (suggested by several alterations of carbohydrate metabolism and triglyceride levels), and endocrine pancreas (since hyperinsulinemia together with impaired glucose tolerance indicate β-cell functional compromise unable to cope with the enhanced demand for insulin due to IR). This evidence concerns the gene INS and hyperinsulinism.