LAGE3 and infection: After 3 h of infection, more callose deposition was observed in mutants of atl9, fbs1, pcc1, and pdf1.2 compared to Col-0 controls, while very little callose deposition was detected in 35S:ATL9, as shown in Figure 7A. Six hours post infection, callose deposition largely increases in all mutants tested, but more callose deposition can be observed in Col-0.