With treating the leukemic cell line with the purified asparaginase, our results revealed that the carcinoma cell line reached cell death up to 83.05% with the highest concentration of the purified enzyme (84 IU/mL) which is equal to about double the MIC50; respectively, leukemic cells require a high concentration of asparagine to keep viable [64]. The gene discussed is ASPG; the disease is carcinoma.