Strikingly, ChIP-Seq analysis using the melanoma cell line WM 9 revealed that HDAC2 inhibition leads to an enrichment of H3K27ac at one specific promoter/enhancer element that is located 55.4 kb upstream of the BRN3A TSS within the proximal promoter of the RNF219 gene. The gene discussed is POU4F1; the disease is melanoma.