Importantly, the addition of OX40/TLR3/9 immunotherapy to SBRT resulted in local depletion of Tregs and reduced Treg-associated gene expression (FoxP3), reduced the density of tumor-associated macrophages and suppressed macrophage-associated gene expression (IL-8), suppressed exhausted T cell-associated gene expression (CTLA4), and induced an increase in circulating IL-7 concentrations compared to dogs treated with SBRT alone. The gene discussed is CTLA4; the disease is neoplasm.