Taken together, because of the pleiotropic effects of pRB in angiogenesis, migration, drug-resistance as well as lysosome functions in GBM cells, we can hypothesize that the siTRPML2-mediated reduction in pRB1 protein expression, can also contribute to VEGFA/VEGFR2 inhibition, Notch 2 activation, increased migration and DOX-resistance reported inTRPML2-silenced GBM cell lines. The gene discussed is PRB1; the disease is glioblastoma.