Here, metformin treatment in human breast (MCF-7), cervical cancer (HeLa), and murine melanoma (B16F10) inhibited anoikis resistance, via reduced ATP, increased cellular stress (Figure 6), and the expression of CHOP-transcriptional factor inherent in pro-apoptotic UPR and misfolded protein accumulation. This evidence concerns the gene DDIT3 and cervical cancer.