Of the other 20 samples that were found negative by the ICT AgTK, 10 samples (numbers 21–30) were found positive for antibodies against Dengue virus NS1 protein using the SD Bioline antigen detection test kit, but negative for IgM and IgG for Dengue fever, leptospirosis and scrub typhus by the respective IFA assays (Table S1). Here, CD40LG is linked to leptospirosis.