To examine the role of FGFRL1 in PCa cells under conditions that mimicked the tumor microenvironment in vitro, PC3M control-KD and FGFRL1-KD cells were cultured in an organotypic 3D cell culture system, supporting physiologically relevant cell–cell and cell–matrix interactions and promoting epithelial differentiation. The gene discussed is FGFRL1; the disease is posterior cortical atrophy.