For an additional validation of the results of the LC-MS/MS measurement, protein expression of HK2 and LDHA were assessed in RPMI-8226, LP-1 and OPM-2 cells and an additional MM cell line U266, as well as in the bone marrow stromal cell line HS-5 in normoxia (21% O2), in acute hypoxia (1% O2, 1 day) and chronic hypoxia (1% O2, 7 days). This evidence concerns the gene LDHA and Miyoshi myopathy.