These types of alterations are not detectable in WT mice up to 16 months, except for a tendency for increased activation of astrocyte and microglia with age, as detected by GFAP and Iba1 reactivity, respectively, but at a level much lower than that found in AD mice (Figure 2K,L; Supplementary Figures S2, S3F,G and S14K,L). This evidence concerns the gene AIF1 and Alzheimer disease.