The involvement of different ceramide synthase isoforms in the synthesis of Cer with specific chain length allowed us to manipulate two distinct pools of ceramide species (18-carbon chain length Cer and 16-carbon chain length Cer) through electroporation-mediated long-term shRNA silencing of individual CerS synthases in the gastrocnemius muscle of mice with HFD-induced insulin resistance. The gene discussed is TLCD3B; the disease is Insulin resistance.