As the viral mimic pre-treatment of both astrocyte cell models resulted in reduced ZIKV load, we chose to investigate the downstream expression of antiviral (IFN-β) and pro-inflammatory (TNF-α) cytokines, along with the known anti-ZIKV host protein, viperin, in pre-treated astrocytes in the presence or absence of ZIKV infection. This evidence concerns the gene IFNB1 and Zika virus infectious disease.