To assess the ability of either the main antiviral cytokines, IFN-β (type I) and IFN-λ (type III), or viral mimic activators of early antiviral innate immunity towards viral infection in our ZIKV-susceptible astrocyte cells, HASTR/ci35 cells were pre-treated with IFNs or dsRNA/dsDNA analogues for 18 h prior to infection with ZIKV (PRVABC59 strain). Here, IFNB1 is linked to viral infectious disease.