Positive markers, as described by the Minimal Information for Studies of Extracellular Vesicles (MISEV) [28], include tetraspanins, CD81 (Class 1a), CD9 (Class 1b), and tumour susceptibility gene 101 (TSG101; Class 2a) and were detected in EV isolates from control, NAFL, and NASH subjects (Figure 2c). This evidence concerns the gene CD81 and non-alcoholic fatty liver.