ATF4 and steatosis: Furthermore, tetracycline as doxycycline and minocycline could play a role in the progression of steatosis by enhancing oxidative stress generation, via the activating transcription factor 4 (ATF4), which induces ROS generation by means of CYP2E1 upregulation, and the inhibition of the mammalian target of rapamycin (mTOR) [80].