To study how twist1a+ and kras+ affected tumor growth and liver tumor metastasis over the long term, all zebrafish groups (twist1a+, kras+, twist1a+/kras+, and wild-type) were treated with 20 μg/mL Dox and 1μg/mL 4-OHT for 4 weeks. This evidence concerns the gene KRAS and Neoplasm of the liver.