Histological analysis of twist1a+/kras+ zebrafish revealed the presence of HP, HCA, HCC, and metastatic HCC in some fish (4 wpi: 1/9; 2/9; 4/9; 2/9, respectively), whereas the twist1a+/kras+/LPS zebrafish presented more severe metastatic HCC (4 wpi: 3/19; 1/19; 4/19; 11/19, respectively). Here, KRAS is linked to hepatocellular carcinoma.