Upon oral administration, the Map4k4 siRNA, encapsulated in galactosylated trimethyl chitosan-cysteine (GTC)/tripolyphosphate (TPP) or GTC/HA NPs, significantly decreased the expression of TNF-α in colonic cells and related parameters in the DSS-induced colitis in a mouse model [91,92]. The gene discussed is TNF; the disease is colitis.