ICOSLG and neoplasm: Similar amounts of both cell types were detected in lungs metastasized with either B16-ICOSL-low or B16-ICOSL-high cells (Figure 4C), suggesting that the metastasis process, in this setting, is not influenced by recruitment of M-MDSC and Tregs in premetastatic niches and finally, the percentages are in line with those detected in the lungs of tumor-free mice (Supplementary Figure S3).