Some of these TLRs have been implicated in the pathogenesis of NAFLD and NASH, such as TLR2, which usually forms heterodimers with TLR6, and senses peptidoglycan; TLR4, which binds myeloid differentiation factor 2 protein (MD-2), and senses lipopolysaccharides (LPS); and the intracellular TLR9, implicated in sensing unmethylated DNA as the mitochondrial DNA (mtDNA) [69,70,71,72]. This evidence concerns the gene TLR4 and metabolic dysfunction-associated steatohepatitis.