Then, in evaluating the regulation of CSC properties by the MSI1/miR-671-5p/TRAF2 axis, we found that CSC markers in GBM cells with overexpression of miR-671-5p alone were significantly reduced compared with those in cells with TRAF2 overexpression, and this effect was rescued by co-overexpression of MSI1 and miR-671-5p, consistent with the mRNA level quantification results (Figure 4d,e). This evidence concerns the gene MSI1 and glioblastoma.