Correspondingly, we found that the activation of AKT and IR increased the most with insulin stimulation or the treatment with Diane-35 and metformin combined, but the phosphorylation of AKT and IR was decreased when AR was overexpressed or GLUT4 was underexpressed, suggesting that the negative regulation of IR/AKT associated with PCOS was influenced by hyperandrogenism, which can be controlled by Diane-35 and metformin. Here, INS is linked to hyperandrogenism.