Of the 26 genes with de novo splice altering missense and synonymous variants in ASD probands, 6 were previously reported in Simons Foundation for Autism Research Initiative Gene database (SFARI Gene) as having an association with ASD (CHKB, CACNA2D1, ERBB2IP, SYNGAP1, TCF4, USP45, S2 Table). Here, SYNGAP1 is linked to autism.