Our sterol analysis from murine PC models also substantiates that sterol metabolites, especially cholesterol and DHC, are high in the pancreas of KPC mice harboring activated KrasG12D and Trp53R172H compared to the normal pancreas and KC mice (Figure 4), which indicates recapitulation of human pancreatic tumor sterol levels in mice models of PC. This evidence concerns the gene CALCA and pachyonychia congenita.