miR-133b inhibited ARFGEF1 protein expression in cervical cancer cells, and overexpression of ARFGEF1 weakened the inhibitory effects of miR-133b on the proliferation, invasion, migration, and clone formation ability of C33A cells, suggesting miR-133b/ARFGEF1 axis repressed biological functions of cervical cancer cells Moreover, we verified that ARFGEF1 expression was severely elevated in patients with cervical cancer. The gene discussed is ARFGEF1; the disease is cervical carcinoma.