miR-133b inhibited ARFGEF1 protein expression in cervical cancer cells, and overexpression of ARFGEF1 weakened the inhibitory effects of miR-133b on the proliferation, invasion, migration, and clone formation ability of C33A cells, suggesting miR-133b/ARFGEF1 axis repressed biological functions of cervical cancer cells Moreover, we verified that ARFGEF1 expression was severely elevated in patients with cervical cancer. This evidence concerns the gene ARFGEF1 and cervical cancer.