To determine whether pyruvate supplementation promotes tumor cell survival upon DNA damage through its binding to SSRP1, we supplemented SSRP1‐depleted U251 or U87 cells rescued with rSSRP1 WT or pyruvate‐binding deficient mutant R54A with or without pyruvate, followed by the treatment of etoposide. Here, SSRP1 is linked to neoplasm.