As an initial proof of concept, multicellular spheroids were prepared (average diameter of 375 μm and a standard deviation of 8 μm) using CT26 colon carcinoma cells, labeled with CellTracker Green, captured in the device, then imaged in the absence of fluid flow or with perfusion (constant flow rate of 5 μL/min) over 70 h with media containing APC Annexin V. In the absence of fluid flow, the normalized fluorescence intensity of the spheroid decreased by 70% over 40 h, reaching a plateau that remained consistent from the 40 to 70 h time points (Figure 4 and Supplementary Figure 2). Here, ANXA5 is linked to colon carcinoma.