Furthermore, immunization subsequent to disease establishment mediated a protective effect, downmodulating IL-6 and IL-12 production and upregulating levels of IL-10 in animals with induced arthritis, reducing only IL-6, TNF-α, IFN-γ, and IL-17 cytokines without changing levels of IL-10 or increasing the frequency of Tregs in the spleen of experimental autoimmune encephalomyelitis (EAE) mice (71). Here, IL6 is linked to arthritic joint disease.