108 Somatic mutations were identified in NKX2-5 and its molecular partners, TBX5 and GATA4, as well as the transcription factor HEY2, in formalin-fixed tissues taken from a collection of hearts with atrial septal defect, 109 ventricular septal defect, and atrioventricular canal defect. The gene discussed is TBX5; the disease is Abnormal cardiac septum morphology.