Examples of differentially regulated immune genes after lice alone infection included interleukins (il17d, il7r, il2rb, il12b, and il6st), chemokines (ackr3, ackr4, ccr2, ccr6, ccr7, ccr9, cxcl12, ccl13, ccl17, ccl20), metallopeptidases (adam9, adam17, adamts8, adamts12, adamts17, adamts18, adamts20, mmp11, and mmp15), transcription factors (gata3, stat1), and apoptosis (rnf213, map3k11, litaf, scarb2, tagap, lgals4, and bok) (Supplementary File 5, sub-table T1). This evidence concerns the gene ACKR4 and infection.