To further investigate the role of Mir221/222 in obesity and diabetes, we crossed male Tg (Adipoq-cre) mice and female Mir221tm2/y C57BL/6JJcl mice and generated male Mir221/222flox/y and male Mir221/222AdipoKO littermates. This evidence concerns the gene MIR221 and obesity due to melanocortin 4 receptor deficiency.