H460 EVs also modestly downregulated E-cadherin and ZO-1, consistent with the flow cytometry data shown in Fig. 4A. The only cancer EVs that did not appear to alter E-cadherin and ZO-1 expression or localization were A549 EVs, suggesting that the increased permeability imposed by A549 EVs (Fig. 3) is likely mediated by another mechanism. This evidence concerns the gene TJP1 and cancer.