CDH1 and cancer: H460 EVs also modestly downregulated E-cadherin and ZO-1, consistent with the flow cytometry data shown in Fig. 4A. The only cancer EVs that did not appear to alter E-cadherin and ZO-1 expression or localization were A549 EVs, suggesting that the increased permeability imposed by A549 EVs (Fig. 3) is likely mediated by another mechanism.