We showed that EVs from the high secretion rate cancer cell lines (Calu6 and H358) consistently had a higher propensity to induce various cancerous phenotypes in recipient cells, including invasion (Fig. 2), epithelial barrier disruption (Fig. 3), and E-cadherin downregulation (Fig. 4) than the low secretion rate cancer cell lines (A549 and H460). The gene discussed is CDH1; the disease is cancer.