Similar significant gene sets as week 16 of gestation were also identified at week 20 (FDR p < 0.05): SPTB versus HTERM (n = 3705), SPTB versus PPROM (n = 70) and PPROM versus HTERM (n = 8469). This evidence concerns the gene SERPINH1 and preterm premature rupture of the membranes.