Targeted deep sequencing analyses revealed efficient gene editing in the AAVS1 and SUPT4H1 loci reaching up to mean of 93.6 and 95.7% total indel efficiencies (mean of 29.5% in-frame, 64.1% out-of-frame mutation for Non-HD, and 27.9% in-frame, 67.9% out-of-frame mutation for Q57 HD iPSC-NPCs) in Non-HD and Q57 HD iPSC-NPCs subsequently (Supplementary Fig. 1a and Supplementary Table 1). Here, SUPT4H1 is linked to Huntington disease.