CXCL12 and neoplasm: We sequentially extracted the tumor specimens with Nonidet P-40, sodium deoxycholate (DOC), DNase, and sodium dodecyl sulfate (SDS) and analyzed the lysates by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting with anti-KRT19 and anti-CXCL12 antibodies.