Neither by Western blot nor by immunoprecipitation from different cell lines (Fig. 1B, C and also 1205Lu melanoma cells, HCC827 lung carcinoma, K562 chronic myelogenous leukemia and MDA-MB231 breast cancer cells; data not shown) we were able to detect a signal for Usp27xS (around 50 kDa). This evidence concerns the gene USP27X and melanoma.