CYTH1 and infection: Second, SUVECs were transfected with the indicated GBF1 plasmid [GBF1-FLAG, GBF1-(1–885)-FLAG, GBF1-(1–698)-FLAG, and GBF1-Sec7-FLAG] for 48 h, and cells were cotreated with CSFV (multiplicity of infection [MOI] of 1) and BFA (100 nmol/L).