Therefore, the functional part of the training set was defined using rare (described once in only one cancer database) or absent variants whereas the non-functional variants used for the training set were issued from the CSD, that includes p53 variants coexisting in four major independent cancer mutation databases [36] (see Materials and Methods) (Figure 1 and Supplementary Table S1 available online at http://bib.oxfordjournals.org/). The gene discussed is TP53; the disease is cancer.