However, given our previous study showing that, rather than the burden of circulating EVs, it was the parsing of EVs populations for their parental origin which allowed to discriminate between responders and non-responders to ICI [25], we performed the correlations by considering the percentage of PD1+ EVs and PDL-1+ EVs released from both tumor and non-tumor cells (immune cells) in the multivariate analysis. The gene discussed is PDCD1; the disease is neoplasm.