The determination of whether PD1+ EVs were differently released by MM patients with normal or high LDH level, measured before immunotherapy, evidenced a statistically increased percentage of these EVs only when they were released from melanoma and dendritic cells in patients with high LDH, from a median value of 7.47 to 14.95% and from 8.32 to 15.88%, respectively (Supplemental Fig. S6A). This evidence concerns the gene PDCD1 and melanoma.