For the pilot study of 3 ESCC patients and 3 healthy volunteers (Fig. 1A), the isolated exosomes from either saliva or cell lysate were confirmed by TEM (Fig. S1A) and immunoblot using antibodies against specific exosomal markers (Alix, TSG101, CD63, CD9) and Calnexin, an intracellular protein that is not present in exosomes (Fig. S1B). Here, CANX is linked to esophageal squamous cell carcinoma.